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KMID : 0357920070410060380
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Korean Journal of Pathology
2007 Volume.41 No. 6 p.380 ~ p.386
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Analysis of Microsatellite Instability in Ovarian Epithelial Cancer
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Sol Mi-Young
Kim Jee-Yeon
Shin Dong-Hoon
Choi Kyung-Un
Kang Hyun-Jeong
Kim Ick-Doo
Seo Soon-Jung
Choi Hyo-Seon
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Abstract
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Background : The aim of this study was to clarify the incidence and role of microsatellite instability (MSI) in sporadic ovarian epithelial cancers (OEC). We investigated the MSI status and mismatch repair (MMR) protein expression in OEC.
Methods : MSI was examined by fluorescence- based polymerase chain reaction using five NCI panel markers (BAT25, BAT26, D2S123, D5S346 and D17S250) in 46 cases of OEC. Immunohistochemistry (IHC) for hMLH1 and
hMSH2 was performed.
Results : Seven cases (15.2%) exhibited high-frequency MSI (MSIH), one exhibited low-frequency MSI (MSI-L), and the remaining 38 demonstrated microsatellite stability (MSS). MSI-H in OEC was not associated with histologic grade, FIGO stage, tumor size, mitoses or histologic type. Loss of expression of either hMLH1 or hMSH2 was observed
in 4 of the 7 (59.3%) MSI-H cases, whereas 4 of the 39 (10.3%) MSI-L or MSS tumors revealed loss of expression of MMR proteins. The sensitivity and specificity of immunohistochemistry for hMLH1 and hMSH2 were 57.1% and 89.7%.
Conclusions : Our data suggest that a genetic defect in the MMR system might play a role in the carcinogenesis of a minor subset of sporadic OEC however, immunohistochemical testing for hMLH1 and hMSH2 cannot accurately
determine microsatellite instability status in OEC.
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KEYWORD
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Microsatellite instability, Ovarian neoplasm, hMLH1, hMSH2, Immunohistochemistry
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